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ADVANCED INSTRUMENTATION TECHNIQUES BP811ET
UNIT -01
- Nuclear Magnetic Resonance spectroscopy Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications. Mass Spectrometry- Principles, Fragmentation, Ionization techniques- Electron impact, chemical ionization, MALDI, FAB, Analyzers -Time of flight and Quadrupole, instrumentation, applications.
UNIT -02
- Thermal Methods of Analysis: Principles, instrumentation and applications of Thermogravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC). X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray Crystallography, rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation and applications.
UNIT -03
- Calibration and validation- as per ICH and USFDA guidelines. Calibration of following Instruments: Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC.
UNIT -04
Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immunoassay. Extraction techniques: General principle and procedure involved in the solid phase extraction and liquid-liquid extraction.
UNIT -05
- Hyphenated techniques- LC-MS/MS, GC-MS/MS, HPTLC-MS.