INSTRUMENTAL METHODS OF ANALYSIS BP701T

UNIT -01

UV Visible spectroscopy: Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications- Spectrophotometric titrations, Single component and multi component analysis.
Fluorimetry: Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications.

IR spectroscopy: Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations. Instrumentation- Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.
Flame Photometry– Principle, interferences, instrumentation and applications.
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications.
Nephelo-turbidimetry- Principle, instrumentation and applications.

Introduction to chromatography:
Adsorption and partition column chromatography- Methodology, advantages, disadvantages and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications.
Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications.

Gas chromatography – Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications.
High performance liquid chromatography (HPLC)- Introduction, theory, instrumentation, advantages and applications.

Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications.
Gel chromatography- Introduction, theory, instrumentation and applications.
Affinity chromatography- Introduction, theory, instrumentation and applications.