PUBLIC HEALTH PHARMACY

PHARMACEUTICAL BIOTECHNOLOGY BP605T

UNIT -01

  • Brief introduction to Biotechnology with reference to Pharmaceutical Sciences. 
  • Enzyme Biotechnology- Methods of enzyme immobilization and applications. 
  • Biosensors- Working and applications of biosensors in Pharmaceutical Industries. 
  • Brief introduction to Protein Engineering. 
  • Use of microbes in industry. Production of Enzymes- General consideration – Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase. 
  • Basic principles of genetic engineering. 

UNIT -02

  • Study of cloning vectors, restriction endonucleases and DNA ligase. 
  •  Recombinant DNA technology. 
  • Application of genetic engineering in medicine. Application of r DNA technology and genetic engineering in the production of: 
  •  i) Interferon 
  •  ii) Vaccines- hepatitis- B 
  •  iii) Hormones-Insulin. Brief introduction to PCR. 

UNIT -03

 

  • Types of immunity- humoral immunity, cellular immunity. 
  • Structure of Immunoglobulins. 
  • Structure and Function of MHC. 
  • Hypersensitivity reactions, Immune stimulation and Immune suppressions. 
  • General method of the preparation of bacterial infections, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity. 
  • Storage conditions and stability of official vaccines. 
  • Hybridoma technology- Production, Purification and Applications, Blood products and Plasma Substitutes.

UNIT -04

  • Immuno-blotting techniques- ELISA, Western blotting, Southern blotting. Genetic organization of Eukaryotes and Prokaryotes. 

  • Microbial genetics including transformation, transduction, conjugation, plasmids and transposons. 

  • Introduction to Microbial biotransformation and applications. 

  • Mutation: Types of mutation/mutants.

UNIT -05

  • Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring. 
  • Large scale production fermenter design and its various controls. 
  • Study of the production of – Penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin. 
  • Blood Products: Collection, Processing and Storage of whole human blood, dried human plasma, plasma Substitutes.